PowerSoil DNA Isolation Kit

Giving You The Power To Do More With Soil



Protocol

12888

Specifications

Format
Method
Binding Capacity
Through-put
Time
Equipment Required
Silica Spin Filter Tubes
Bead Beating
Up to 20 µg per filter
1 - 24 samples
30 minutes
Vortex Adapter


Kit Components

Component
12888-50
12888-100
PowerBead Tubes
Solution C1
Solution C2
Solution C3
Solution C4
Solution C5
Solution C6
Spin Filters In Tubes
Collection Tubes
50
1 x 3.3 ml
1 x 14 ml
1 x 11 ml
1 x 72 ml
1 x 27.5 ml
1 x 6 ml
50
200
100
1 x 6.6 ml
1 x 28 ml
1 x 22 ml
1 x 144 ml
1 x 55 ml
1 x 12 ml
100
400



Storage Conditions

Store at room temperature for up to 1 year.

Description:
Catalog #:
50 Preps       STOCK!
12888-50
100 Preps
12888-100


Description

The PowerSoil DNA Isolation Kit* is comprised of a novel and proprietary method for isolating genomic DNA from environmental samples. The kit is intended for use with environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, and manure. Other more common soil types have also been used successfully with this kit. The isolated DNA has a high level of purity allowing for more successful PCR amplification of organisms from the sample. PCR analysis has been performed to detect a variety of organisms including bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces).

The PowerSoil DNA Kit distinguishes itself from Mo Bio's UtraClean Soil DNA Isolation Kit with a NEW humic substance/brown color removal procedure. This new procedure is effective at removing PCR inhibitors from even the most difficult soil types.

Environmental samples are added to a bead beating tube for rapid and thorough homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic DNA is captured on a silica membrane in a spin column format. DNA is then washed and eluted from the spin column. DNA is ready for PCR analysis and other downstream applications.
Features and Benefits

More Sample Types - Processes more soil types including difficult environmental samples.

More PCR Results - PCR inhibition was not observed in any of the samples tested.

More Time Savings - Purifies DNA from up to 250 mg of soil in 30 minutes.

More Purity - Gives high quality DNA free of PCR inhibitors including humic substances.

More Clarity - No visible brown coloration was seen in any of the samples tested.

Potential Applications - Biodefense, Soil-Borne Pathogens, Agriculture, Zoology.