PowerSoil DNA Isolation Kit
Giving You The Power To Do More With Soil |
|
Protocol
12888
Specifications
Format Method Binding Capacity Through-put Time Equipment Required | Silica Spin Filter Tubes Bead Beating Up to 20 µg per filter 1 - 24 samples 30 minutes Vortex Adapter |
Kit Components
Component
|
12888-50
|
12888-100
|
PowerBead Tubes
Solution C1
Solution C2
Solution C3
Solution C4
Solution C5
Solution C6
Spin Filters In Tubes
Collection Tubes |
50 1 x 3.3 ml 1 x 14 ml 1 x 11 ml 1 x 72 ml 1 x 27.5 ml 1 x 6 ml 50 200 | 100 1 x 6.6 ml 1 x 28 ml 1 x 22 ml 1 x 144 ml 1 x 55 ml 1 x 12 ml 100 400 |
Storage Conditions
Store at room temperature for up to 1 year.
|
Description: |
Catalog #: |
50 Preps STOCK! |
12888-50 |
100 Preps |
12888-100 |
|
Description
The PowerSoil DNA Isolation Kit* is comprised of a novel and proprietary method for isolating genomic DNA from environmental samples. The kit is intended for use with environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, and manure. Other more common soil types have also been used successfully with this kit. The isolated DNA has a high level of purity allowing for more successful PCR amplification of organisms from the sample. PCR analysis has been performed to detect a variety of organisms including bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces).
The PowerSoil DNA Kit distinguishes itself from Mo Bio's UtraClean Soil DNA Isolation Kit with a NEW humic substance/brown color removal procedure. This new procedure is effective at removing PCR inhibitors from even the most difficult soil types.
Environmental samples are added to a bead beating tube for rapid and thorough homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic DNA is captured on a silica membrane in a spin column format. DNA is then washed and eluted from the spin column. DNA is ready for PCR analysis and other downstream applications.
|
Features and Benefits
More Sample Types - Processes more soil types including difficult environmental samples.
More PCR Results - PCR inhibition was not observed in any of the samples tested.
More Time Savings - Purifies DNA from up to 250 mg of soil in 30 minutes.
More Purity - Gives high quality DNA free of PCR inhibitors including humic substances.
More Clarity - No visible brown coloration was seen in any of the samples tested.
Potential Applications - Biodefense, Soil-Borne Pathogens, Agriculture, Zoology.
|
|
|