Taq DNA Polymerase with ThermoPol Buffer Catalog # Size Concentration M0267S    STOCK! 400 units 5,000 units/ml M0267L 2,000 units 5,000 units/ml Download: Technical Bulletin| Isolated from a recombinant source Supplied with 10X Reaction Buffer Description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations. Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 Applications: PCR Primer extension Colony PCR Long PCR (>5 kb) Reagents Supplied: ThermoPol Reaction Buffer Pack Enzyme Properties 3´ to 5´ Exonuclease: No 5´ to 3´ Exonuclease: Yes Strand Displacement: No Error Rate: 285 x10-6 bases Heat Inactivation: No Molecular Weight: Theoretical: 94,000 daltons Specific Activity: 40,000 units/mg Reaction & Storage Conditions Reaction Conditions: 1X ThermoPol Reaction Buffer Pack 1X ThermoPol Reaction Buffer Pack: 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1 % Triton X-100 pH 8.8 @ 25°C Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA. Concentration: 5,000 units/ml Storage Conditions: 10 mM Tris-HCl 100 mM KCl 1 mM Dithiothreitol 0.1 mM EDTA 50% Glycerol 0.5% Tween-20 0.5% NP-40 pH 7.4 @ 25°C Storage Temperature: -20°C Quality Control 3' to 5' Exonuclease Activity: No detectable 3' → 5' nuclease activity was observed when 20 units of Taq DNA Polymerase with ThermoPol Buffer was incubated with substrates containing either 3' extensions or blunt ends. Endonuclease Activity: Incubation of a 50 μl reaction containing 20 units of Taq DNA Polymerase with ThermoPol Buffer with 1 μg of ΦX174 RF I DNA for 4 hours at 75ºC resulted in < 10% conversion to RFII as determined by agarose gel electrophoresis. 5 kb Lambda PCR: 25 cycles of PCR amplification of 5 ng Lambda DNA with 2.5 units of Taq DNA Polymerase in the presence of 200 µM dNTPS, 0.2 µM primers and 1X Standard Taq Reaction Buffer resulted in a yield of 200 ng specific product. Quality control values for a specific lot can be found on the datacard which accompanies each product.