Protein Marker, Broad Range (2-212 kDa)
Catalog # Size Concentration
P7702S   STOCK! 150 mini-gel lanes  
P7702L 750 mini-gel lanes  



10-20% SDS-PAGE


Description:
Protein Marker, Broad Range is a mixture of purified proteins with known amino acid sequences. They are resolved to 13 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coomassie Blue R-250 (1). Two bands (BSA, MW 66.4 kDa and Triosephosphate isomerase, MW 26.6 kDa) are at double intensity to serve as reference points.

Usage notes:

  1. Mix Protein Marker. Bring the desired amount of the Protein Marker (7 µl for mini-gels and 15 µl for full length gels) over to a separate tube.
  2. Heat the Marker to 95-100°C for 3-5 minutes.
  3. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.