Spe I Catalog # Size Concentration R0133S   STOCK! 500 units 10,000 units/ml R0133L 2,500 units 10,000 units/ml R0133M 2,500 units 50,000 units/ml Recognition Site: Source: A E. coli strain that carries the Spe I gene from Sphaerotilus species (ATCC 13923). Reagents Supplied: NEBuffer 2 BSA Enzyme Properties Activity in NEBuffers: NEBuffer 1: 75% NEBuffer 2: 100% NEBuffer 3: 25% NEBuffer 4: 75% When using a buffer other than the optimal (supplied) NEBuffer, it may be necessary to add more enzyme to achieve complete digestion. Methylation Sensitivity: dam methylation: Not sensitive dcm methylation: Not sensitive CpG methylation: Not sensitive Heat Inactivation: 65°C for 20 minutes Survival in a Reaction: Minimum units to digest 1 µg of substrate DNA in 16 hours: 0.50 unit(s) Reaction & Storage Conditions Reaction Conditions: 1X NEBuffer 2 Supplemented with 100 μg/ml Bovine Serum Albumin Incubate at 37°C. 1X NEBuffer 2: 10 mM Tris-HCl 50 mM NaCl 10 mM MgCl2 1 mM Dithiothreitol pH 7.9 @ 25°C Unit Definition: One unit is defined as the amount of enzyme required to digest 1 µg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Concentration: 10,000 units/ml and 50,000 units/ml Unit Assay Substrate: Adenovirus-2 DNA Storage Conditions: 10 mM Tris-HCl 50 mM KCl 1 mM Dithiothreitol 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C Storage Temperature: -20°C Diluent Compatibility: Diluent A Notes General notes: Cleaves to leave a 5´ CTAG extension which can be efficiently ligated to DNA fragments generated by Avr II, Nhe I, or Xba I.