Xba I
Cloned At NEBRecombinant SourceNEBuffer 2BSA37Heat InactivateddamPass Blue White Selection Assay
Catalog # Size Concentration
R0145S   STOCK! 3,000 units 20,000 units/ml
R0145L 15,000 units 20,000 units/ml
R0145T 3,000 units 100,000 units/ml
R0145M 15,000 units 100,000 units/ml


Recognition Site:

TCTAGA


Source:
A E. coli strain that carries the Xba I gene from Xanthomonas badrii (ATCC 11672).

Reagents Supplied:
NEBuffer 2
BSA


Enzyme Properties


Activity in NEBuffers:
NEBuffer 1:0%
NEBuffer 2:100%
NEBuffer 3:75%
NEBuffer 4:75%

When using a buffer other than the optimal (supplied) NEBuffer, it may be necessary to add more enzyme to achieve complete digestion.

Methylation Sensitivity:
dam methylation: Blocked by overlapping
dcm methylation: Not sensitive
CpG methylation: Not sensitive


Heat Inactivation:
65°C for 20 minutes

Survival in a Reaction:
Minimum units to digest 1 µg of substrate DNA in 16 hours: 0.13 unit(s)


Reaction & Storage Conditions


Reaction Conditions:
1X NEBuffer 2
Supplemented with 100 μg/ml Bovine Serum Albumin
Incubate at 37°C.

1X NEBuffer 2:
10 mM Tris-HCl
50 mM NaCl
10 mM MgCl2
1 mM Dithiothreitol
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (dam-/Hind III digest) in 1 hour at 37°C in a total reaction volume of 50 µl.

Concentration:
20,000 units/ml and 100,000 units/ml

Unit Assay Substrate:
λ DNA (dam-/Hind III digest)

Storage Conditions:
10 mM Tris-HCl
50 mM NaCl
1 mM Dithiothreitol
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Storage Temperature:
-20°C

Diluent Compatibility:
Diluent A