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R0145S STOCK! |
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3,000 units |
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20,000 units/ml |
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R0145L |
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15,000 units |
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20,000 units/ml |
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R0145T |
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3,000 units
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100,000 units/ml |
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R0145M |
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15,000 units |
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100,000 units/ml |
Recognition Site:
Source: A E. coli strain that carries the Xba I gene from Xanthomonas badrii (ATCC 11672).
Reagents Supplied: NEBuffer 2 BSA
Enzyme Properties
Activity in NEBuffers:
NEBuffer 1: | | 0% | NEBuffer 2: | | 100% | NEBuffer 3: | | 75% | NEBuffer 4: | | 75% |
When using a buffer other than the optimal (supplied) NEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
Methylation Sensitivity:
dam methylation: Blocked by overlapping | dcm methylation: Not sensitive | CpG methylation: Not sensitive |
Heat Inactivation:
65°C for 20 minutes
Survival in a Reaction:
Minimum units to digest 1 µg of substrate DNA in 16 hours: 0.13 unit(s)
Reaction & Storage Conditions
Reaction Conditions:
1X NEBuffer 2
Supplemented with 100 μg/ml Bovine Serum Albumin
Incubate at
37°C.
1X NEBuffer 2:
10 mM Tris-HCl
50 mM NaCl
10 mM MgCl2
1 mM Dithiothreitol
pH 7.9 @ 25°C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (dam-/Hind III digest) in 1 hour at 37°C in a total reaction volume of 50 µl.
Concentration:
20,000 units/ml and 100,000 units/ml
Unit Assay Substrate:
λ DNA (dam-/Hind III digest)
Storage Conditions:
10 mM Tris-HCl
50 mM NaCl
1 mM Dithiothreitol
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Storage Temperature:
-20°C
Diluent Compatibility:
Diluent A
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