• Obtain the entire 5’ and 3’ cDNA ends
• Begin with as little as 25 ng of poly A⁺ or 50 ng of total RNA
• Use first-strand cDNA directly in RACE PCR—no adaptor ligation required

The SMART™ RACE (Rapid Amplification of cDNA Ends) cDNA Amplification Kit is an elegant method to incorporate synthetic adaptors into both the 5’ and 3’ ends of cDNA during synthesis. The result is a powerful method for cloning complete cDNAs that offers several advantages:

Unprecedented ease of use
With our SMART RACE method, only a single tube is needed to perform the two-step procedure. Minimal handling of both your RNA sample and the synthesized cDNA is required. Total hands-on time is only four hours.

Requires only 50 ng of total RNA
Our SMART RACE method allows you to utilize small samples including biopsies, tissue dissections, needle aspirations, embryonic and rare disease tissues. This optimized protocol significantly reduces non-specific background, essential when handling very small sample sizes.

Specific enrichment for 5’ ends
We have designed a specialized SMART™ Oligo that preferentially hybridizes to 5’ ends of the cDNA being synthesized. Using this SMART Oligo, our procedure enriches cDNA pools for 5’ sequences, thus increasing the likelihood you will clone the entire sequence of your gene or the upstream regulatory regions.

No RNA pretreatment required
Our SMART RACE method requires no RNA pretreatment. This protocol works with total RNA, as well as with samples that may be contaminated with genomic DNA.

Powerful combination of hot-start PCR enzymes
We have optimized this kit for use with Advantage™ 2 Polymerase. BD Advantage 2 Polymerase combines TITANIUM™ Taq DNA polymerase, a small amount of proofreading polymerase and TaqStart™ Antibod