• Rapid PCR-based walking in genomic DNA
• Ideal for obtaining exon-intron junctions, promoters, or any other regulatory element
• Rapidly determine unknown sequences

GenomeWalker™ Kits provide a simple, PCR-based method for walking upstream or downstream in genomic DNA from a known sequence, such as an expressed sequence tag (EST). GenomeWalker allows you to take individual steps of up to 6 kb in genomic DNA using long-distance PCR. Walks can be extended simply by taking multiple steps using new primers based on the sequence obtained in previous steps.

Species-specific kits
The GenomeWalker™ Human, Mouse, and Rat Kits contain four premade libraries of adaptor-ligated, species-specific genomic DNA fragments. These libraries are constructed from highly pure genomic DNA of high molecular weight. The DNA is digested separately with four different restriction enzymes and ligated to the specially designed GenomeWalker™ Adaptor. Each kit contains the reagents necessary for up to 20 walks with each library, and 150 primary and 300 nested PCR reactions.

GenomeWalker™ Universal Kit
The GenomeWalker™ Universal Kit allows you to construct GenomeWalker libraries from the genome of any species. The Universal Kit contains reagents sufficient to generate libraries from three different samples and to perform 80 walks.

For all kits, you must provide the set of gene-specific primers and a mix of thermostable DNA polymerases suitable for long-distance PCR (LD PCR). We recommend the Advantage™ Genomic Polymerase Mix or PCR Kit.

Applications
• Genomic DNA walking
• Exon-intron junction determination
• Sequence discovery
• Gene cloning and analysis
• Cloning kits